Thanks for posting. In order to get you the best information here I moved your post to our "Small Business Discussion" board so other people can add their opinions!
Can you tell me about your business? What is the goal of your emails? (Asking them to buy?)
What subject lines have you tried? How large of a list are you sending to?
Sending the correct message to the correct people at the correct time.
Without knowing anything about what you're trying to do its kinda hard to share an opinion.
Whats your story ?
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